Tuesday, March 20, 2007

Basics of Microarray analysis

Bio CC wiki for microarray analysis( In Korean) : http://expressome.org/

A sequence of microarray experiment
  1. cDNA Spotting
  2. RNA preparation
  3. Hybridization
  4. Scanning
  5. Statistics

Problems in microarray data analysis

RNA extracts might be different according to which environment and when they were extracted. It's related to false positive signals (DEG).

Platform difference : different set of DEG could be determined between cDNA microarray chip which measure expression pattern from two samples and oligonucleotide chip which measures from single sample (absolute expression level).